One of examples of a ATP -generating reaction medium “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance, described by us is concrete reaction as Glucose + membrane - redox potentials three - state line system +Oxygen ➜ Carbon dioxide + Water + Energy Released (ATP +Heat energy, during which have been occurred the reaction of reduction of oxygen to water with the passage of electron to oxygen through the reduction reaction in right side of equation. ATP can be produced by the three main pathways in eukaryotes are (1) glycolysis, (2) the citric acid cycle -oxidative phosphorylation, and (3) beta-oxidation. In the form of the overall process of oxidizing glucose to carbon dioxide, the combination of pathways 1 and 2, known as cellular respiration, produces about 30 equivalents of ATP from each molecule of glucose , all these reactions are inseparable parts of a reaction medium as “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance, described by us. It should be say that ATP have been played the role in this process as one of four "monomers" required in the synthesis of RNA., promoted by RNA polymerases, a similar process occurs in the formation of DNA, except that ATP is first converted to the deoxyribonucleotide dATP, like many condensation reactions in nature, DNA replication and DNA transcription also consume ATP, by using these complex reactions as Glycolysis, Oxidative deamination, Betta-oxidation followed by Krebs cycle to acceptors, transporting protons across a membrane ,within the inner mitochondrial membrane, coenzyme Q10 (Q) carries both electrons and protons by a redox cycle Q accepts two electrons and two protons, it becomes reduced to the ubiquinol form (QH2); when QH2 releases two electrons and two protons, it becomes oxidized back to the ubiquinone (Q) form lead to the formation of proton gradient within a the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance in the human body, described by us. In the context of biochemical reactions, the P-O-P bonds are frequently referred to as high-energy bonds, the hydrolysis of ATP into ADP and inorganic phosphate releases 30.5 kJ/mol of enthalpy, with a change in free energy of 3.4 kJ/mol, a typical intracellular concentration of ATP is 1–10 μmol per gram of tissue in the course of aerobic metabolism all these processes have been conducted by using a reaction medium as “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance, described by us.