2-Dimensional Electrical Resistivity Imaging Survey profiles of length 320 and 300m was carried out for Substructural investigations in Ovia North East Local Government Area, Benin- City, Edo State, Nigeria. Wenner–Schlumberger Array and Wenner- Alpha were used for the field data acquisition using Pasi Earth Resistivity Meter. The field data were processed using RES2DINV, 2-Dimensional electrical tomography data processing software to obtain 2- Dimensional true resistivity of portion of the substructure chosen for visualization. The subsurface stratigraphy is as follows; Top soil with resistivity and depth values ranging from 616 - 1163Ωm and 0.0-20.7m, dry sand with resistivity and depth values ranging from 1394- 4861Ωm and 13.8-35.9m, and Silty sand with resistivity and depth values ranging from 4136- 10734Ωm and 35.4-54.3m. The 2-D resistivity imagery reveal the absence of an aquiferous zone, also the absence of clay formation considering the range of 1-120ohm meter usually associated with clay in the study area could be the best area for building constructions.