Characterization of polyphenolic extract and rich fraction from Oldenlandia corymbosa (L)

Medicinal plants are often used as raw materials to extract active ingredients used in the synthesis of various medicines. The focus of this research is on the isolation and direct use of active medicinal ingredients or the development of semi-synthetic drugs or the active screening of natural products to obtain synthetic pharmacologically active compounds. The aqueous methanol extract (1 mL, 1 mg/mL) was mixed thoroughly with 1 mL of 50% Folin-Ciocalteu reagent. 1 mL of 2% Na2CO3, and centrifuged at 13400X g for 5 min. The polyphenolic rich extract was placed carefully in pre-coated aluminum silica gel 60 F, Merck F 254 using a micro capillary tube. Irradiating the samples with UV light, H2O2 was added to a mM concentration of 2.5mmol/L. The reaction volume was maintained in the lid of a polyethylene micro centrifuge tube placed directly on the surface of the transilluminator (8000 mW/cm) at 300 nm. Untreated pBR322 plasmid was included as a control for each gel electrophoresis. The extract development was viewed under UV240nm and 360 nm. The polyphenolic-rich fraction of the Oldenlandia corymbosa L. ensured the highest flavonoid content and exhibited the strongest antidiabetic activity.