Detection of LasB AND AprA genes of pseudomonas Aeruginosa isolated from different infection in Hilla province

Pseudomonas aeruginosa is a significant cause of bloodstream infections acquired in healthcare settings. The microorganism synthesizes two main proteases: alkaline protease A (AprA) and elastase B (LasB). This study gathered one hundred samples from clinical cases of people suffering from wound, ear, urinary tract, and blood and burn infections. The phenotypic properties of Pseudomonas aeruginosa were ascertained using the gram-staining approach, which involved observing the formation of pigments following incubation at a temperature of 37°C. Analysis of biological constituents and mechanisms. The outcome derived from Pseudomonas aeruginosa utilizing Las B: The allocation of isolates was as follows: 10% (5 isolates) were obtained from cases of otitis media, 40% (20 isolates) were obtained from burn infections, 30% (15 isolates) were obtained from wound infections, 8% (5 isolates) were obtained from urinary tract infections, and 12% (6 isolates) were obtained from blood samples. The distribution of alkaline protease A (AprA) in isolates from different sources is as follows: The allocation of isolates was as follows: The distribution of isolates was as follows: 8% from otitis media (4 isolates), 50% from infection of burn (25 isolates), 26% from infections of wound (13 isolates), 8% from urinary tract infections (4 isolates), and 8% from blood (4 isolates). Conclusion: Out of 100 clinical samples, analysis of the virulence gene showed that 50 samples of Pseudomonas aeruginosa contained both the AprA gene and the Las B gene.  The genes provide the genetic instructions for producing the elastase enzyme, recognized for its exceptional effectiveness in protein analysis and tissue death. On the other hand, the AprA gene can increase the synthesis of pyocyanin in this bacteria