This research was carried out to explore the effect employee motivation on job performance in public institutions. The general objective was to establish the relationship between employee motivation and job performance in Gasabo District, while specific objectives include assessing different forms of motivation used in Gasabo district, to evaluate the role of employee motivation on Job performance in Gasabo district and establishing the relationship between employee motivation and job performance at Gasabo district. The research was carried out in Gasabo District head office located in Kacyiru sector. The target population was 51 employees of Gasabo District and sample size was also 51 respondents. After analyzing the data, the finding indicated that employee motivation, have positive effect on job performance. The study found out that there are different forms of motivation in Gasabo District like salary, bonus, promotion, and benefits/allowances. It also found that the role of motivation lead to commitment, timeliness, goals achievement and not tending to leave the institution - employee turnover as results of motivation. The respondents’ employees at Gasabo District agreed that there is a relationship between employee motivation and job performance hence confirming that job performance is affected by motivational forms. Hence concluded that when employee is motivated, his or her job performance will increase and as well result to organizational success. Under this study some weaknesses were found and the researcher recommended to the employer to promote employees fairly, provide effectively bonuses without basing on the overall organizational or departmental performance but on individual performance, recommend to the government and other institutions to invest in motivating employees and ensure their satisfaction for better job performance.