Modern instructional technology holds the potential to deliver effective learner-centered, personalized education for non-traditional and non-residential students worldwide. With artificial intelligence (AI) becoming an integral part of our daily lives, it is transforming education and reshaping how we learn, communicate, interact, and work. Our previous research showcased the positive impact of interactive learning activities on students' performance in an anatomy and physiology course. The objective of this research paper is to underscore the significance of integrating ChatGPT’s artificial intelligence (AI) and interactive learning activities into a General Biology course. To accomplish this objective, we developed and implemented ChatGPT’s AI and interactive learning activities within the General Biology curriculum. We assessed students; performance and perceptions through various metrics, including class attendance, discussion forums, computerized homework, proctored exams, and the use of classroom technologies. In summary, our study emphasizes the importance of incorporating ChatGPT’s AI and interactive learning activities into education. Our findings reveal that students not only enjoyed but also appreciated the innovative approach, as evident by the positive feedback in course surveys. Integrating ChatGPT’s AI and interactive learning activities into curriculum ensures that students are not only well-prepared for their academic pursuits but also for future workforce development.