NATURAL OCCURRING COMPOUND SODIUM BORATE SHOWED MARKED ANTIMYCOTIC ACTION ON CLINICAL CASES OF ONYCHOMYCOSIS AND AGAINST ASSOCIATED FUNGAL SPECIES BY IN-VITRO TESTING IN-VITRO

Aim: The objectives of this study was to study the therapeutic antimycotic action of natural occurring compound borate on onychomycosis patients and also to observe in-vitro antimycotic properties of borate on fungal species isolated from clinical onychomycosis cases. Methods: This study was conducted from Aug.2017 to March 2018 involving 3 patients and 3 samples. Clinical sample (of clipped nails) were collected aseptically and preliminary microscopic examination using 20% potassium hydroxide (KOH) was conducted. Further, isolation and identification of mycotic species was performed using standard protocol using Sabouraud dextrose agar base media (SDA) and dermatophyte test agar media (DTM). Isolated fungi were tested for antifungal susceptibility against 3% concentration of borate testing using semisolid agar method. The percentage of inhibition was calculated by measuring the size of colonies after 7days and 14 days. Negative control with addition of borate and positive control in adding miconazole nitrate at the rate of 100 microgram/milliliter (Himedia) in the growth medium were include. Patients were treated with shudha tankana malhar. Result: All 3 patients of onychomycosis showed 80-100% recovery. The dermatophyte isolate belonged to Trichophyton mentagrophyte, showed 100% inhibition against sodium borate as compared to positive control i.e. miconazole nitrate after 7th and 14th day of post inoculation. Conclusion: Sodium borate can be inexpansive therapeutic option for treating cases onychomycosis, which is otherwise not easy to cure by standard drug therapy.