Role of long non coding rnas (hot air) as a biomarker in breast cancer

In recent years, increasing numbers of long non-coding RNAs (lncRNAs) have been identified as playing an important role in breast cancer progression. Here, the long non Coding RNA (HOTAIR) expression was studied as a potential biomarker in primary localized breast cancer. Patient were divided into two groups,   the first is the study group, includes 50 patients with primary localized breast cancer while the second control group includes 50 normal females of matched age to the study group. Samples of whole blood were collected by vein puncture in a gel Vacutainer tube and processed for tumor markers study, Carcinoembryonic antigen (CEA) and Cancer antigen (CA-15.3) and the long non Coding RNA (HOTAIR).Tissue samples obtained from study group for histopathological examination and determination of Immunohistochemistry, Estrogen receptors (ER), Progesterone Receptors (PR) and Human Epidermal Receptor-2 (HER2)which have been  assessed as risk factors for breast cancer.Our results showed a high significant difference in the expression of Lnc-HOTAIRby two folds up-regulation in breast cancer patients compared to control group. Also we founded no significant association between any of the tumor receptors, and Lnc_HOTAIR gene expression.