The annual short-day plant known as Artemisia annua is a member of the Asteraceae family of living plants. It has a stem that is upright and brownish or violet brown in colour. Despite the fact that it is possible for plants to reach a height of 200 centimetres when they are grown in cultivation, the plant itself does not have any hair and grows naturally between 30 and 100 centimetres tall. A. annua leaves are around three to five centimetres in length and are sliced deeply into two or three leaflets. These leaflets are divided into two or three leaflets. The present investigation focuses on determining the antibacterial capabilities of the fractions that are extracted from Artemisia annua leaves, as well as the bioactive components that are present in the extract. As part of the preparation for FTIR analysis, Peak (Wave number cm-ˡ), (Type of Intensity, Bond and Functional group assignment) were 675.09(Strong, C-Cl, alkyl halides), 692.44(Strong, C-Cl, alkyl halides), 738.74(Strong, =C–H, Alkenes), 813.96(Strong, =C–H, Alkenes), 974.05(Strong, =C–H, Alkenes), 1008.77(Strong, C-F, alkyl halides), 1049.28(Strong, C-F, alkyl halides), 1093.64(Strong, C-F, alkyl halides), 1232.51(Strong, C-F, alkyl halides), 1276.88(Strong, C-F, alkyl halides), 2922.16 (Strong, C-H, Alkane), 1276.88(Strong, C-F, alkyl halides), 1606.70(Bending, N-H, Amide), 1647.21(Variable, C=C, Alkene). The metabolites of Artemisia annua exhibited significant activity against Klebsiella pneumoniae (27.84 ±0.45).