This paper aimed to analyze the impact of biodiversity loss and environmental degradation in the southern part of Bangladesh. The study employed qualitative research methodology drawing in data from a wide range of sources through unstructured interviews, key informant interviews, and secondary literature. Using a comprehensive thematic analysis approach, the study identified valuable insights into the challenges faced by the region and proposed opportunities for sustainable conservation and management of biodiversity and ecosystems. The causes of biodiversity loss include deforestation, unsustainable agriculture, pollution, overexploitation of resources, climate change, population growth, and limited awareness. These causes ultimately lead to ecosystem disruption and environmental degradation in the selected area and pose threatening challenges to the habitat and livelihoods of the local communities. Nevertheless, despite having limited resources, conflicting interests, weak governance, and lack of awareness, conservation and management strategies involve protected areas, sustainable practices, community engagement, strengthening governance, and environmental education. Moreover, opportunities exist in policy integration, international collaboration, community-based conservation, sustainable livelihoods, and research and innovation. A sustainable and resilient ecosystem can be achieved in the southern part of Bangladesh by addressing these challenges and capitalizing on the opportunities.