Currently a major problem related to virtual commissioning activities is the lack of precision between the virtual model and the physical model, due to the difficulties related to the construction on the factory floor of the previously generated 3D model. The proposed methodology of calibration, calibration and validation of the digital twin can be applied to different production segments, and this is a competitive differential in relation to automation solutions strictly developed for the automotive industry, also allowing professional qualification at a distance with virtual reality that the project will make it possible as an unfolding. The objective of this research is to develop a methodology that makes use of the digital twin calibration and virtual commissioning to digitally validate robotic mechatronic cells, and minimize the time of installation and implementation of the project on the “factory floor” in the context of I4.0.